Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MMS2 antibody (ab101475)

Key features and details

  • Rabbit polyclonal to MMS2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MMS2 antibody
    See all MMS2 primary antibodies

  • Description

    Rabbit polyclonal to MMS2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human MMS2 aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab113304)

  • Positive control

    • This antibody gave a positive signal in mouse testis tissue lysate and in the following whole cell lysates: HL60; HeLa; U2OS; Jurkat; NIH 3T3; PC12. ICC/IF: HeLa cells.

  • General notes

     This product was previously labelled as Ube2v2, MMS2

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab101475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.

  • Tissue specificity

    Detected in placenta, colon, liver and skin. Detected at very low levels in most tissues.

  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 1 alpha 25 dihydroxyvitamin D3 inducible antibody
    • DDVit 1 antibody
    • DDVIT1 antibody
    • EDAF-1 antibody
    • EDAF1 antibody
    • EDPF-1 antibody
    • EDPF1 antibody
    • Enterocyte differentiation associated factor EDAF 1 antibody
    • Enterocyte differentiation promoting factor antibody
    • Enterocyte differentiation-associated factor 1 antibody
    • Enterocyte differentiation-promoting factor 1 antibody
    • Methyl methanesulfonate sensitive 2 antibody
    • MMS 2 antibody
    • MMS2 antibody
    • MMS2 homolog antibody
    • UB2V2_HUMAN antibody
    • UBE2V 2 antibody
    • UBE2V2 antibody
    • Ubiquitin conjugating enzyme E2 variant 2 antibody
    • Ubiquitin conjugating enzyme E2v2 antibody
    • Ubiquitin-conjugating enzyme E2 variant 2 antibody
    • UEV 2 antibody
    • UEV2 antibody
    • Vitamin D3 inducible protein antibody
    • Vitamin D3-inducible protein antibody
    see all

Images

  • Western blot - Anti-MMS2 antibody (ab101475)
    Western blot - Anti-MMS2 antibody (ab101475)
    All lanes : Anti-MMS2 antibody (ab101475) at 1 µg/ml

    Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 38 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-MMS2 antibody (ab101475)
    Immunocytochemistry/ Immunofluorescence - Anti-MMS2 antibody (ab101475)
    ICC/IF image of ab101475 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101475, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml.
  • Western blot - Anti-MMS2 antibody (ab101475)
    Western blot - Anti-MMS2 antibody (ab101475)
    All lanes : Anti-MMS2 antibody (ab101475) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa
    Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

References (0)

Publishing research using ab101475? Please let us know so that we can cite the reference in this datasheet.

ab101475 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-MMS2 antibody (ab101475)
    Western blot - Anti-MMS2 antibody (ab101475)
    All lanes : Anti-MMS2 antibody (ab101475) at 1 µg/ml

    Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 38 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-MMS2 antibody (ab101475)
    Immunocytochemistry/ Immunofluorescence - Anti-MMS2 antibody (ab101475)
    ICC/IF image of ab101475 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101475, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml.
  • Western blot - Anti-MMS2 antibody (ab101475)
    Western blot - Anti-MMS2 antibody (ab101475)
    All lanes : Anti-MMS2 antibody (ab101475) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa
    Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

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