Anti-Stathmin 1 (phospho S37) antibody (ab47399)
Key features and details
- Rabbit polyclonal to Stathmin 1 (phospho S37)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Stathmin 1 (phospho S37) antibody
See all Stathmin 1 primary antibodies -
Description
Rabbit polyclonal to Stathmin 1 (phospho S37) -
Host species
Rabbit -
Specificity
This antibody is specific for only when phosphorylated at serine 37. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthesized phosphopeptide derived from human Stathmin 1 around the phosphorylation site of serine 37 (P-L-SP-P-P)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinitychromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Stathmin 1 (phospho S37) antibody (ab47399)All lanes : Anti-Stathmin 1 (phospho S37) antibody (ab47399) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with PMA (1ng/ml, 15min)
Lane 2 : Extracts from Jurkat cells untreated
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S37) antibody (ab47399)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47399 at a 1/50 dilution.
Left image: Untreated
Right image: Treated with phosphopeptide -
Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 (phospho S37) antibody (ab47399)ICC/IF image of ab47399 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47399, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
