Recombinant human CD137 protein (Fc Chimera Active) (ab220584)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, ELISA, Flow Cyt
Preparation and Storage
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Images
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SDS-PAGE - Recombinant Human CD137 protein (Fc Chimera) (ab220584)
SDS-PAGE analysis of reduced ab220584 stained overnight with Coomassie Blue. The protein migrates as 55-60 kDa due to glycosylation.
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ELISA - Recombinant Human CD137 protein (Fc Chimera) (ab220584)Immobilized ab220584 at 0.1 μg/mL (100 μL/well) can bind Recombinant human 4-1BBL protein (Fc Chimera Active) (Biotin) (ab246043) with a linear range of 0.4-13 ng/mL.
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ELISA - Recombinant human CD137 protein (Fc Chimera Active) (ab220584)Immobilized ab220584 at 1 μg/mL (100 μL/well) can bind Recombinant human 4-1BBL protein (Fc Chimera Active) (Biotin) (ab246043) with a linear range of 0.2-3 ng/mL.
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Functional Studies - Recombinant Human CD137 protein (Fc Chimera) (ab220584)Loaded ab220584 on Protein A Biosensor, can bind Recombinant human 4-1BBL protein (Fc Chimera Active) (Biotin) (ab246043) with an affinity constant of 1.3 nM as determined in BLI assay.
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Flow Cytometry - Recombinant Human CD137 protein (Fc Chimera) (ab220584)Flow Cytometry assay shows that ab220584 can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.1 μg/mL.
