Anti-Nucleolin antibody [EPR7951] (ab134164)
![Anti-Nucleolin antibody [EPR7951] (ab134164)](https://www.abcam.com/ps/products/134/ab134164/Images/ab134164-341556-ab134164KOWBGDBIPAL.jpg "Anti-Nucleolin antibody [EPR7951] (ab134164)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7951] to Nucleolin
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Nucleolin antibody [EPR7951]
See all Nucleolin primary antibodies -
Description
Rabbit monoclonal [EPR7951] to Nucleolin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to residues near the N-terminal in Human Nucleolin (P19338).
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Positive control
- K562, HuT-78, HeLa, and Jurkat cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 2.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7951 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nucleolin antibody [EPR7951] (ab134164)All lanes : Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/10000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : Nucleolin knockout HeLa whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 76 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab134164 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab134164 was shown to specifically react with Nucleolin in wild-type HeLa cells as signal was lost in Nucleolin knockout cells. Wild-type and Nucleolin knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab134164 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Nucleolin antibody [EPR7951] (ab134164)](https://www.abcam.com/ps/products/134/ab134164/Images/ab134164-148451-anti-nucleolin-antibody-epr7951-western-blot.jpg)
Western blot - Anti-Nucleolin antibody [EPR7951] (ab134164)All lanes : Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/1000 dilution
Lane 1 : K562 cell lysates
Lane 2 : HuT-78 cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 76 kDa
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![OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] (ab134164)](https://www.abcam.com/ps/products/134/ab134164/Images/ab134164-148450-anti-nucleolin-antibody-epr7951.jpg)
OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] (ab134164) -
![Anti-Nucleolin antibody [EPR7951] (ab134164)](https://www.abcam.com/ps/products/134/ab134164/Images/ab134164-4-benefits-of-recombinant-antibodies.png)
Anti-Nucleolin antibody [EPR7951] (ab134164)
