Anti-Nucleolin antibody [3G4B2] (ab14966)
![Anti-Nucleolin antibody [3G4B2] (ab14966)](https://www.abcam.com/ps/products/14/ab14966/Images/ab14966-22234-anti-nucleolin-antibody-3g4b2-western-blot.jpg "Anti-Nucleolin antibody [3G4B2] (ab14966)")
Key features and details
- Mouse monoclonal [3G4B2] to Nucleolin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Nucleolin antibody [3G4B2]
See all Nucleolin primary antibodies -
Description
Mouse monoclonal [3G4B2] to Nucleolin -
Host species
Mouse -
Specificity
Recognizes human nucleolin, MW 100kD and an additional protein, MW 95kDa in HeLa nuclear extract. The clone number has been updated from (4i51) to (3G4B2) both clone numbers name the same antibody clone. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Nuclear matrix extract from MDCK cells (dog).
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Positive control
- WB: HeLa nuclear extract. IP: MDCK total cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 30% Glycerol
Supplied as a liquid in 100µl of culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%. -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
3G4B2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Nucleolin antibody [3G4B2] (ab14966)Anti-Nucleolin antibody [3G4B2] (ab14966) at 1/5000 dilution + Hela nuclear lysate
Developed using the ECL technique.
Predicted band size: 85 kDa
HeLa nuclear extract was probed with anti-nucleolin (1:5000 dilution).
