Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µl 268 032 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Anillin antibody [CL0303] (ab211872)

Key features and details

Mouse monoclonal [CL0303] to Anillin
Suitable for: ICC/IF, IHC-P, WB
Reacts with: Human
Isotype: IgG1

Product name

Anti-Anillin antibody [CL0303]
See all Anillin primary antibodies
Description

Mouse monoclonal [CL0303] to Anillin
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Anillin aa 300-500.
Database link: Q9NQW6

Run BLAST with Run BLAST with
Epitope

Binds to an epitope located within the peptide sequence LRGRFDKGNIWSAEK (438-452 aa) as determined by overlapping synthetic peptides.
Positive control
- U251 cell line lysate; Human colorectal cancer, Human lung cancer, Human testis, Human colon, Human stomach, Human tonsil and Human liver tissues; HeLa, A431, MCF7, U2OS and U251 cells.
General notes

Marker for Nucleus without Nucleoli
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL0303
Isotype

IgG1
Research areas

Western blot - Anti-Anillin antibody [CL0303] (ab211872)

All lanes : Anti-Anillin antibody [CL0303] (ab211872)

All lanes : U-251 cells, transfected with control siRNA

Predicted band size: 124 kDa

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (ab211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized U251 cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (ab211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human tonsil tissue labeling Anillin with ab211872 at 1/50 dilution.
Western blot - Anti-Anillin antibody [CL0303] (ab211872)

Anti-Anillin antibody [CL0303] (ab211872) at 1/500 dilution + U251 cell line lysate

Predicted band size: 124 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (ab211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized U2OS cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.
Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (ab211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized MCF7 cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.
Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (ab211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized A431 cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.
Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (ab211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized HeLa cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (ab211872)

Negative control: Immunohistochemical analysis of paraffin-embedded, formalin fixed Human liver tissue labeling Anillin with ab211872 at 1/50 dilution shows absence of staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (ab211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human stomach tissue labeling Anillin with ab211872 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (ab211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human colon tissue labeling Anillin with ab211872 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (ab211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human testis tissue labeling Anillin with ab211872 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (ab211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human lung cancer tissue labeling Anillin with ab211872 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (ab211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human colorectal cancer tissue labeling Anillin with ab211872 at 1/50 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com