Anti-TET1 antibody - C-terminal (ab191698)
Key features and details
- Rabbit polyclonal to TET1 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TET1 antibody - C-terminal
See all TET1 primary antibodies -
Description
Rabbit polyclonal to TET1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseHumanIHC-P MouseHumanWB MouseRatHuman
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Immunogen
Synthetic peptide within Human TET1 (C terminal). The exact sequence is proprietary. 18 amino acid peptide. The immunogen is located with amino acids 2030 - 2080.
Database link: Q8NFU7 -
Positive control
- Western blot: Human, mouse and rat testis; human bladder. IHC-P: Human and mouse testis. ICC/IF: HeLa and EL4 cell lines.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TET1 antibody - C-terminal (ab191698)All lanes : Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/ml
Lane 1 : Mouse testis
Lane 2 : Rat testis
Lane 3 : Human testis
Lane 4 : Human bladder
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 235 kDa
Additional bands at: 55-72 kDa (possible non-specific binding)
Exposure time: 1 minute8% SDS gel
Running condition: 130V, 1.5 hours.
Transfer condition: Wet, 250mA, 2 hrs (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight.
Primary antibody incubation: room temperature for 1 hour in 5% NFDM/TBST.
Secondary antibody incubation: room temperature for 1 hour.
Washing condition: 5 mL TBST, 4 x 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TET1 with ab191698 at 1 µg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on human testis is observed. DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody - C-terminal (ab191698)Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) HeLa cells labeling TET1 with ab191698 at 20 µg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 3 x 3 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (ab191698)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling TET1 with ab191698 at 1 µg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on mouse testis is observed. DAB staining.
Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.
Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 2 X 5 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody - C-terminal (ab191698)Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) EL4 cells (mouse T-lymphocyte cell line) labeling TET1 with ab191698 at 20 µg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on EL4 cell line. The nuclear counter stain is DAPI (blue).
Blocking condition: 5% BSA, room temperature, 1 hour.
Washing condition: PBS, 3 x 3 minutes.
