Anti-Tbx2 antibody (ab33298)
Key features and details
- Rabbit polyclonal to Tbx2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Tbx2 antibody
See all Tbx2 primary antibodies -
Description
Rabbit polyclonal to Tbx2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog, Xenopus laevis
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Tbx2.
Read Abcam's proprietary immunogen policy (Peptide available as ab35983.) -
Positive control
- This antibody gave a positive signal in the following Human tissue lysates: Kidney; Lung; Heart
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab33298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Target
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Function
Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. -
Tissue specificity
Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary. -
Sequence similarities
Contains 1 T-box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 448779 Dog
- Entrez Gene: 6909 Human
- Entrez Gene: 21385 Mouse
- Omim: 600747 Human
- SwissProt: Q863A2 Dog
- SwissProt: Q13207 Human
- SwissProt: Q60707 Mouse
- Unigene: 16322 Dog
see all -
Alternative names
- FLJ10169 antibody
- HGNC:11597 antibody
- T box 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tbx2 antibody (ab33298)Formalin-fixed paraffin-embedded mouse bone marrow section stained with ab33298 Tbx2 antibody at 1/500 dilution.
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Western blot - Anti-Tbx2 antibody (ab33298)All lanes : Anti-Tbx2 antibody (ab33298) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 74 kDa -
Immunohistochemistry (Frozen sections) - Anti-Tbx2 antibody (ab33298)Image courtesy of Mr Xu Jun by Abreviewab33298 staining Tbx2 in mouse heart and somatopleure tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with methanol. Samples were then blocked with 5% serum for 2 hours at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An undiluted Cy2®-conjugated Donkey anti-rabbit IgG was used as secondary antibody.
Protocols
References (4)
ab33298 has been referenced in 4 publications.
- Durbin AD et al. Selective gene dependencies in MYCN-amplified neuroblastoma include the core transcriptional regulatory circuitry. Nat Genet 50:1240-1246 (2018). PubMed: 30127528
- Lv Y et al. TBX2 over-expression promotes nasopharyngeal cancer cell proliferation and invasion. Oncotarget 8:52699-52707 (2017). PubMed: 28881763
- Liu F et al. TBX2 expression is regulated by PAX3 in the melanocyte lineage. Pigment Cell Melanoma Res 26:67-77 (2013). PubMed: 23020925
- Lüdtke TH et al. Tbx2 controls lung growth by direct repression of the cell cycle inhibitor genes Cdkn1a and Cdkn1b. PLoS Genet 9:e1003189 (2013). PubMed: 23341776
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tbx2 antibody (ab33298)Formalin-fixed paraffin-embedded mouse bone marrow section stained with ab33298 Tbx2 antibody at 1/500 dilution.
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Western blot - Anti-Tbx2 antibody (ab33298)All lanes : Anti-Tbx2 antibody (ab33298) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 74 kDa
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Immunohistochemistry (Frozen sections) - Anti-Tbx2 antibody (ab33298) Image courtesy of Mr Xu Jun by Abreview
ab33298 staining Tbx2 in mouse heart and somatopleure tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with methanol. Samples were then blocked with 5% serum for 2 hours at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An undiluted Cy2®-conjugated Donkey anti-rabbit IgG was used as secondary antibody.
