Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SUFU antibody [EPR23821-101] (ab259975)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23821-101] to SUFU
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SUFU antibody [EPR23821-101]
    See all SUFU primary antibodies

  • Description

    Rabbit monoclonal [EPR23821-101] to SUFU

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HEK-293T, NIH/3T3, PC-12 and LNCaP whole cell lysates. Flow cyt: HAP1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)
    Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)
    All lanes : Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
    Lane 2 : SUFU knockout HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
    Lane 3 : Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 4 : SUFU knockout HEK293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 5 : LNCaP (human prostate carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lanes 1-5: Merged signal (red and green). Green - ab259975 observed at 53 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    Lanes 1-2: ab253183 Anti-SUFU antibody [EPR23821-101] was shown to react with SUFU in HAP1 cells in Western blot. Loss of signal was observed when SUFU knockout sample was used. Wild-type and SUFU knockout samples were subjected to SDS-PAGE.

     Lanes 3-4: ab253183 Anti-SUFU antibody [EPR23821-101] was shown to specifically react with SUFU in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267282 (knockout cell lysate ab257718) was used.  Wild-type and SUFU knockout samples were subjected to SDS-PAGE. 

    ab253183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4° overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Flow Cytometry - Anti-SUFU antibody [EPR23821-101] (ab259975)
    Flow Cytometry - Anti-SUFU antibody [EPR23821-101] (ab259975)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line, Right) / SUFU KO HAP1 (Left) cells labelling SUFU with ab259975 at 1/500 dilution (0.1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)
    Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)
    All lanes : Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysates were used in this WB.

    Exposure time: 26 seconds.

     

  • Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)
    Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)
    Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution + LNCaP (human prostate carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysates were used in this WB.

    Exposure time: 37 seconds.

     

  • Anti-SUFU antibody [EPR23821-101] (ab259975)
    Anti-SUFU antibody [EPR23821-101] (ab259975)

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