Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

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Anti-SUFU antibody [EPR23821-101] (ab259975)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23821-101] to SUFU
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-SUFU antibody [EPR23821-101]
See all SUFU primary antibodies
Description

Rabbit monoclonal [EPR23821-101] to SUFU
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HAP1, HEK-293T, NIH/3T3, PC-12 and LNCaP whole cell lysates. Flow cyt: HAP1 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23821-101
Isotype

IgG
Research areas

Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)

All lanes : Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
Lane 2 : SUFU knockout HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
Lane 3 : Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 4 : SUFU knockout HEK293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 5 : LNCaP (human prostate carcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Lanes 1-5: Merged signal (red and green). Green - ab259975 observed at 53 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

Lanes 1-2: ab253183 Anti-SUFU antibody [EPR23821-101] was shown to react with SUFU in HAP1 cells in Western blot. Loss of signal was observed when SUFU knockout sample was used. Wild-type and SUFU knockout samples were subjected to SDS-PAGE.

Lanes 3-4: ab253183 Anti-SUFU antibody [EPR23821-101] was shown to specifically react with SUFU in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267282 (knockout cell lysate ab257718) was used. Wild-type and SUFU knockout samples were subjected to SDS-PAGE.

ab253183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4° overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-SUFU antibody [EPR23821-101] (ab259975)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line, Right) / SUFU KO HAP1 (Left) cells labelling SUFU with ab259975 at 1/500 dilution (0.1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)

All lanes : Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Fresh lysates were used in this WB.

Exposure time: 26 seconds.
Western blot - Anti-SUFU antibody [EPR23821-101] (ab259975)

Anti-SUFU antibody [EPR23821-101] (ab259975) at 1/1000 dilution + LNCaP (human prostate carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Fresh lysates were used in this WB.

Exposure time: 37 seconds.
Anti-SUFU antibody [EPR23821-101] (ab259975)

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