HRP Anti-PTEN antibody [Y184] (ab199545)
![HRP Anti-PTEN antibody [Y184] (ab199545)](https://www.abcam.com/ps/products/199/ab199545/Images/ab199545-310949-anti-pten-antibody-y184-hrp-western-blot.jpg "HRP Anti-PTEN antibody [Y184] (ab199545)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y184] to PTEN
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-PTEN antibody [Y184]
See all PTEN primary antibodies -
Description
HRP Rabbit monoclonal [Y184] to PTEN -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab157804) -
Positive control
- WB: MCF7 whole cell lysates.
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General notes
Alternative versions available:
Anti-PTEN antibody [Y184] (ab32199) - Knockout validatedOur RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y184 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-PTEN antibody [Y184] (ab199545)All lanes : HRP Anti-PTEN antibody [Y184] (ab199545) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PTEN knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 47 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesab199545 was shown to recognize PTEN in wild-type HAP1 cells as signal was lost at the expected MW in PTEN knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PTEN knockout samples were subjected to SDS-PAGE. Ab199545 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-PTEN antibody [Y184] (ab199545)](https://www.abcam.com/ps/products/199/ab199545/Images/ab199545-246517-anti-pten-antibody-y184-hrp-western-blot.jpg)
Western blot - HRP Anti-PTEN antibody [Y184] (ab199545)HRP Anti-PTEN antibody [Y184] (ab199545) at 1/5000 dilution + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199545 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-PTEN antibody [Y184] (ab199545)](https://www.abcam.com/ps/products/199/ab199545/Images/ab199545-4-benefits-of-recombinant-antibodies.png)
HRP Anti-PTEN antibody [Y184] (ab199545)
