Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9941-2] to PTEN - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PTEN antibody [EPR9941-2] - BSA and Azide free
    See all PTEN primary antibodies

  • Description

    Rabbit monoclonal [EPR9941-2] to PTEN - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Full length protein corresponding to Human PTEN aa 1 to the C-terminus.
    Database link: P60484

  • Positive control

    • WB: HeLa, MCF7 and 293T cell lysates. IHC-P: Human breast carcinoma and colon tissues.

  • General notes

    ab271924 is the carrier-free version of ab170941. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)
    Western blot - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PTEN knockout HAP1 cell lysate (20 µg)
    Lanes 1 and 2: Merged signal (red and green). Green - ab170941 observed at 47 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170941).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling PTEN with Purified ab170941 at 1:50 dilution (5.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170941).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling PTEN with Purified ab170941 at 1:50 dilution (5.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170941).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling PTEN with Purified ab170941 at 1:50 dilution (5.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170941).
  • Flow Cytometry - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)
    Flow Cytometry - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling PTEN with purified ab170941 at 1/120 dilution(10ug/ml) (red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170941).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PTEN with unpurifed ab170941 at 1/50 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170941).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PTEN with unpurifed ab170941 at 1/50 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170941).
  • Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)
    Anti-PTEN antibody [EPR9941-2] - BSA and Azide free (ab271924)

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