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Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15201(B)] to intestinal alkaline phosphatase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-intestinal alkaline phosphatase antibody [EPR15201(B)]
    See all intestinal alkaline phosphatase primary antibodies

  • Description

    Rabbit monoclonal [EPR15201(B)] to intestinal alkaline phosphatase

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 85.7% homology with ALPPL2 (Alkaline phosphatase placental-like). Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, JAR and Human placenta lysate; Human small intestine tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
    Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
    All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution

    Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human placenta lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 15 seconds


    Blocking and Diluting buffer: 5% NFDM/TBST

    This antibody does not work in Caco-2 cells which should be positive (PMID: 20660763).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

    Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Alkaline Phosphatase with ab186422 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
    Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
    Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/10000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 57 kDa

  • Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
    Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
    All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : Human placenta lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 57 kDa

  • Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
    Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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