Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 243 ₸

Order now and get it on Thursday March 04, 2021

Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15201(B)] to intestinal alkaline phosphatase
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-intestinal alkaline phosphatase antibody [EPR15201(B)]
See all intestinal alkaline phosphatase primary antibodies
Description

Rabbit monoclonal [EPR15201(B)] to intestinal alkaline phosphatase
Host species

Rabbit
Specificity

The immunogen used for this product shares 85.7% homology with ALPPL2 (Alkaline phosphatase placental-like). Cross-reactivity with this protein has not been confirmed experimentally.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, JAR and Human placenta lysate; Human small intestine tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15201(B)
Isotype

IgG
Research areas

Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution

Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human placenta lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 57 kDa

Exposure time: 15 seconds

Blocking and Diluting buffer: 5% NFDM/TBST

This antibody does not work in Caco-2 cells which should be positive (PMID: 20660763).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Alkaline Phosphatase with ab186422 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/10000 dilution + HeLa cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 57 kDa
Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution

Lane 1 : JAR cell lysate
Lane 2 : Human placenta lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 57 kDa
Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com