Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9839] to Acid phosphatase/ACP1
  • Suitable for: WB, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Acid phosphatase/ACP1 antibody [EPR9839]
    See all Acid phosphatase/ACP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9839] to Acid phosphatase/ACP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human Acid phosphatase/ACP1.
    Database link: P24666

  • Positive control

    • Human placenta, HeLa and HepG2 lysates; permeabilized Hela cells.

  • General notes

     This product was previously labelled as Acid Phosphatase

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)
    Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)
    All lanes : Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : ACP1 knockout HEK-293 whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab166896 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab166896 was shown to recognize ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost at the expected MW in ACP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab166896 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)
    Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)
    All lanes : Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size: 18 kDa

  • Flow Cytometry - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)
    Flow Cytometry - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)

    Flow cytometric analysis of permeabilized HeLa cells labeling Acid Phosphatase/ACP1 using ab166896 at 1/10 dilution (red) compared to staining with a rabbi IgG negative control antibody (green).

  • Immunoprecipitation - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)
    Immunoprecipitation - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)
    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166896 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)
    Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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