Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21791] to Acid phosphatase - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free
    See all Acid phosphatase primary antibodies

  • Description

    Rabbit monoclonal [EPR21791] to Acid phosphatase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, K562, HeLa, HCT 116, Jurkat and HepG2 whole cell lysate; Human placenta tissue lysate. IHC-P: Human colon cancer and prostatic hyperplasia tissue. ICC/IF: HeLa and HepG2 cells. Flow Cyt: HeLa cells.

  • General notes

    Ab238913 is the carrier-free version of ab235449. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238913 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)
    Western blot - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)
    All lanes : Anti-Acid phosphatase antibody [EPR21791] (ab235449) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 cell lysate
    Lane 2 : ACP1 knockout HEK-293 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab235449).

    Lanes 1 - 4: Merged signal (red and green). Green - ab235449 observed at 18 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab235449 was shown to react with Acid phosphatase in wild-type HEK-293 cells in western blot with loss of signal observed in ACP1 knockout sample. Wild-type and ACP1 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab235449 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)

    Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labeling Acid Phosphatase with ab235449 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human prostatic hyperplasia (PMID: 26159288) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

  • Flow Cytometry - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)
    Flow Cytometry - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cell line labeling Acid Phosphatase with ab235449 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control, and an unlabeled control (Cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

  • Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)
    Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Acid Phosphatase with ab235449 at 1/100 dilution, followed by ab150077 Alexa-Fluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HepG2 cell line (PMID 26159288) is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain (Red). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

  • Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)
    Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Acid Phosphatase with ab235449 at 1/100 dilution, followed by ab150077 Alexa-Fluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HeLa cell line (PMID 26159288) is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain (Red). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Acid Phosphatase with ab235449 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human colon cancer (PMID: 25811796) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab235449).

     

  • Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)
    Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (ab238913)

© 2021 Astana Biomed Group LLP. All rights reserved.