Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8260] to Granzyme B - Low endotoxin, Azide free
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free
    See all Granzyme B primary antibodies

  • Description

    Rabbit monoclonal [EPR8260] to Granzyme B - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NK-92 cell lysate; IHC-P: Human Hodgkins lymphoma and Human tonsil tissues; Flow Cyt: NK-92 cells;

  • General notes

    ab214443 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)

    This image was made using ab134933 which is the same antibody as ab214443 with BSA and Azide
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry - Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)
    Flow Cytometry - Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)
    This image was made using ab134933 which is the same antibody as ab214443 with BSA and Azide
    Flow Cytometry analysis of NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling Granzyme B with purified ab134933 at 1:70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)

    This IHC data was generated using the same anti-Granzyme B antibody clone, EPR8260, in a different buffer formulation (cat# ab134933).

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human Hodgkin's lymphoma tissue, labelling Granzyme B using unpurified ab134933 at a 1/100 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)
    OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)

    Equilibrium disassociation constant (KD)
    Learn more about KD 

    Click here to learn more about KD

  • Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)
    Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)

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