Anti-Granzyme B antibody [EPR22645-244] (ab255599)
![Anti-Granzyme B antibody [EPR22645-244] (ab255599)](https://www.abcam.com/ps/products/255/ab255599/Images/ab255599-2-anti-granzyme-b-antibody-epr22645-244-flowcytometry-spleen-mouse.jpg "Anti-Granzyme B antibody [EPR22645-244] (ab255599)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22645-244] to Granzyme B
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse
Overview
-
Product name
Anti-Granzyme B antibody [EPR22645-244]
See all Granzyme B primary antibodies -
Description
Rabbit monoclonal [EPR22645-244] to Granzyme B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseWB Mouse
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse splenocyte lysate Flow cyt: Mouse splenocyte cells treated with 2.5ug/ml Concanavalin A for 72h.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22645-244 -
Isotype
IgG -
Research areas
Images
-
Flow Cytometry - Anti-Granzyme B antibody [EPR22645-244] (ab255599)
Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse splenocyte (treated with 2.5ug/ml Concanavalin A for 72h) cells labelling Granzyme B with ab255599 at 1/800 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with anti-CD8 alpha conjugated to Alexa Fluor® 647.Then fixed with 2% PFA for 10min and intracellularly stained with rabbit IgG (Left) or ab255599 (Right).
-
![Western blot - Anti-Granzyme B antibody [EPR22645-244] (ab255599)](https://www.abcam.com/ps/products/255/ab255599/Images/ab255599-1-anti-granzyme-b-antibody-epr22645-244-western-blot-splenocytes-mouse.jpg)
Western blot - Anti-Granzyme B antibody [EPR22645-244] (ab255599)All lanes : Anti-Granzyme B antibody [EPR22645-244] (ab255599) at 1/1000 dilution
Lane 1 : Untreated mouse splenocyte lysate
Lane 2 : Mouse spelenocyte (treated with 2.5µg/ml Concanavalin for 72 hours ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 27 kDa
Observed band size: 27,65 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsThe expression of Granzyme B is elevated in stimulated splenocytes. Granzyme B forms a complex with its protease inhibitor. The molecular weight observed is consistent with what has been described in the literature (PMID: 8663264, PMID: 17116752, PMID: 21919028, PMID: 9774654).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
-
![Anti-Granzyme B antibody [EPR22645-244] (ab255599)](https://www.abcam.com/ps/products/255/ab255599/Images/ab255599-3-benefits-of-recombinant-antibodies.png)
Anti-Granzyme B antibody [EPR22645-244] (ab255599)
