Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22645-206] to Granzyme B - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free
    See all Granzyme B primary antibodies

  • Description

    Rabbit monoclonal [EPR22645-206] to Granzyme B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and colon tissues; Mouse lung and spleen tissue IHC-Fr: Mouse spleen tissue; Rat spleen tissue IP: Mouse spleen tissue

  • General notes

    ab255868 is the carrier-free version of ab255598 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab255868 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). Use our conjugation kits for antibody conjugates that are ready

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Granzyme B with ab255598 at 1/3000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes in human tonsil (PMID: 29042640) is observed.

    The section was incubated with ab255598 for 30 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255598).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Granzyme B with ab255598 at 1/3000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes in human colon is observed.

    The section was incubated with ab255598 for 30 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255598).

  • Immunohistochemistry (Frozen sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)
    Immunohistochemistry (Frozen sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling ab255598 with Granzyme B at 1/200 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. The nuclear counterstain was DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255598).

  • Immunohistochemistry (Frozen sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)
    Immunohistochemistry (Frozen sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat spleen tissue labeling ab255598 with Granzyme B at 1/200 dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution. The nuclear counterstain was DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor 488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255598).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Granzyme B with ab255598 at 1/3000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes in mouse lung (PMID: 23642129) is observed.

    The section was incubated with ab255598 for 30 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255598).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Granzyme B with ab255598 at 1/3000 dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes in mouse spleen (PMID: 7697916) is observed.

    The section was incubated with ab255598 for 30 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255598).

  • Immunoprecipitation - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)
    Immunoprecipitation - Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)

    Granzyme B was immunoprecipitated from 0.35 mg mouse spleen cells (treated with 2.5ug/ml Concanavalin A for 72h) whole cell lysate with ab255598 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab255598 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Mouse spleen cells (treated with 2.5ug/ml Concanavalin A for 72h) whole cell lysate 10μg

    Lane 2: ab255598 IP in mouse spleen cells (treated with 2.5ug/ml Concanavalin A for 72h) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255598 in mouse spleen cells (treated with 2.5ug/ml Concanavalin A for 72h) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255598).

  • Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)
    Anti-Granzyme B antibody [EPR22645-206] - BSA and Azide free (ab255868)

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