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Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8260] to Granzyme B - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Granzyme B antibody [EPR8260] - BSA and Azide free
    See all Granzyme B primary antibodies

  • Description

    Rabbit monoclonal [EPR8260] to Granzyme B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human Hodgkins lymphoma. Human tonsil tissues; Flow Cyt: NK-92 cells.

  • General notes

    ab226162 is the carrier-free version of ab134933. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226162 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human Hodgkin's lymphoma tissue, labelling Granzyme B using unpurified ab134933 at a 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

  • Flow Cytometry - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
    Flow Cytometry - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

    Flow Cytometry analysis of NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling Granzyme B with purified ab134933 at 1:70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

  • OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
    OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

    Equilibrium disassociation constant (KD)
    Learn more about KD 

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134933).

  • Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
    Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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