Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Granzyme B antibody [EPR8260] (ab134933)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8260] to Granzyme B
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Granzyme B antibody [EPR8260]
    See all Granzyme B primary antibodies

  • Description

    Rabbit monoclonal [EPR8260] to Granzyme B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Granzyme B aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: NK-92 cell lysate; IHC-P: Human Hodgkins lymphoma and Human tonsil tissues; Flow Cyt: NK-92 cells;

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Granzyme B antibody [EPR8260] (ab134933)
    Western blot - Anti-Granzyme B antibody [EPR8260] (ab134933)
    Anti-Granzyme B antibody [EPR8260] (ab134933) at 1/10000 dilution (Purified) + NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • Flow Cytometry - Anti-Granzyme B antibody [EPR8260] (ab134933)
    Flow Cytometry - Anti-Granzyme B antibody [EPR8260] (ab134933)
    Flow Cytometry analysis of NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) cells labeling Granzyme B with purified ab134933 at 1:70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human Hodgkin's lymphoma tissue, labelling Granzyme B using unpurified ab134933 at a 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] (ab134933)
    OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] (ab134933)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Granzyme B antibody [EPR8260] (ab134933)
    Anti-Granzyme B antibody [EPR8260] (ab134933)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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