MCF7 (human breast adenocarcinoma cell line) cells stained for CD47 using ab260419 at 2 µg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot (red).

This data was developed using the same antibody clone in a different formulation containing PBS, BSA and azide (ab260419).

Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cells using ab260419 at 2 µg per 10⁶ cells followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

This data was developed using the same antibody clone in a different formulation containing PBS, BSA and azide (ab260419).

Formalin-fixed paraffin-embedded human prostate carcinoma tissue stained for CD47 using ab260419 at 2 µg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different formulation containing PBS, BSA and azide (ab260419).

Formalin-fixed paraffin-embedded human prostate carcinoma tissue stained for CD47 using ab260419 at 2 µg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different formulation containing PBS, BSA and azide (ab260419).

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab260419.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different formulation containing PBS, BSA and azide (ab260419).