Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8052] to CPM - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CPM antibody [EPR8052] - BSA and Azide free
See all CPM primary antibodies -
Description
Rabbit monoclonal [EPR8052] to CPM - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248972 is the carrier-free version of ab150405. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8052 -
Isotype
IgG -
Research areas
Images
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Anti-CPM antibody [EPR8052] (ab150405) at 1/2500 dilution (purified) + Human placenta tissue lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?This data was developed using ab150405, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CPM antibody [EPR8052] (ab150405) at 1/5000 dilution (purified)
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?This data was developed using ab150405, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab150405, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling CPM with purified ab150405 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-CPM antibody [EPR8052] (ab150405) at 1/1000 dilution (unpurified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human fetal liver tissue lysate
Lane 4 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?This data was developed using ab150405, the same antibody clone in a different buffer formulation.
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This data was developed using ab150405, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling CPM with unpurified ab150405 at a dilution of 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab150405, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling CPM with unpurified ab150405 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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