Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8052] to CPM - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CPM antibody [EPR8052] - BSA and Azide free
    See all CPM primary antibodies

  • Description

    Rabbit monoclonal [EPR8052] to CPM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248972 is the carrier-free version of ab150405. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    Western blot - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    Anti-CPM antibody [EPR8052] (ab150405) at 1/2500 dilution (purified) + Human placenta tissue lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab150405, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    Western blot - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    All lanes : Anti-CPM antibody [EPR8052] (ab150405) at 1/5000 dilution (purified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 62 kDa why is the actual band size different from the predicted?



    This data was developed using ab150405, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    This data was developed using ab150405, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling CPM with purified ab150405 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Western blot - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    Western blot - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    All lanes : Anti-CPM antibody [EPR8052] (ab150405) at 1/1000 dilution (unpurified)

    Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 2 : Human fetal kidney tissue lysate
    Lane 3 : Human fetal liver tissue lysate
    Lane 4 : Human placenta lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 51 kDa
    Observed band size: 62 kDa why is the actual band size different from the predicted?



    This data was developed using ab150405, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    This data was developed using ab150405, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling CPM with unpurified ab150405 at a dilution of 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)

    This data was developed using ab150405, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling CPM with unpurified ab150405 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)
    Anti-CPM antibody [EPR8052] - BSA and Azide free (ab248972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.