Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD47 antibody [EPR23002-67] - BSA and Azide free (ab259273)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23002-67] to CD47 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD47 antibody [EPR23002-67] - BSA and Azide free
    See all CD47 primary antibodies

  • Description

    Rabbit monoclonal [EPR23002-67] to CD47 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Human peripheral blood mononuclear cells and U-937 cells.

  • General notes

    Ab259273 is the carrier-free version of ab256495. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab259273 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23002-67

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CD47 antibody [EPR23002-67] - BSA and Azide free (ab259273)
    Flow Cytometry - Anti-CD47 antibody [EPR23002-67] - BSA and Azide free (ab259273)

    Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma, Left) / U-937 (Human monocyte histiocytic lymphoma, Right) cells labelling CD47 with ab256495 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

    Low expression control: HepG2 (PMID: 25721088, 30415063).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256495).

  • Flow Cytometry - Anti-CD47 antibody [EPR23002-67] - BSA and Azide free (ab259273)
    Flow Cytometry - Anti-CD47 antibody [EPR23002-67] - BSA and Azide free (ab259273)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD47 with ab256495 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256495).

  • Anti-CD47 antibody [EPR23002-67] - BSA and Azide free (ab259273)
    Anti-CD47 antibody [EPR23002-67] - BSA and Azide free (ab259273)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD47 antibody [EPR23002-67] - BSA and Azide free (ab259273)

© 2021 Astana Biomed Group LLP. All rights reserved.