Recombinant human CD48 protein (ab179981)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
-
Product name
Recombinant human CD48 protein
See all CD48 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Human CD244 protein Fc Chimera at 5 μg/ml (100μl/well) can bind Human CD48 with a linear range of 0.1- 4 μg/ml.
-
Purity
> 95 % SDS-PAGE. -
Endotoxin level
1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
QGHLVHMTVVSGSNVTLNISESLPENYKQLTWFYTFDQKIVEWDSRKSKY FESKFKGRVR LDPQSGALYISKVQKEDNSTYIMRVLKKTGNEQEWKIK LQVLDPVPKPVIKIEKIEDMDD NCYLKLSCVIPGESVNYTWYGDKRPF PKELQNSVLETTLMPHNYSRCYTCQVSNSVSSKN GTVCLSPPCTLARS -
Predicted molecular weight
23 kDa including tags -
Amino acids
27 to 220 -
Tags
His tag C-Terminus -
Additional sequence information
Mature form.
-
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
-
SDS-PAGE - Recombinant human CD48 protein (ab179981)Human CD48, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
-
Functional Studies - Recombinant human CD48 protein (ab179981)Immobilized Human 2B4, Fc Tag at 5 µg/mL (100 µL/well) can bind Human CD48, His Tag with a linear range of 0.156-2.5 µg/mL. -
SDS-PAGE - Recombinant human CD48 protein (ab179981)SDS-PAGE analysis of ab179981 (DTT-reduced) staining overnight with Coomassie Blue. DTT-reduced protein migrates as 38-45 kDa due to glycosylation.
