Anti-CIT antibody (ab86782)
Key features and details
- Rabbit polyclonal to CIT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CIT antibody
See all CIT primary antibodies -
Description
Rabbit polyclonal to CIT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, from a region within amino acids 1925-1975 of Human CIT (NP_009105.1)
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Positive control
- HeLa whole cell lysate (ab150035) 293T whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CIT antibody (ab86782)All lanes : Anti-CIT antibody (ab86782) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 231 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-CIT antibody (ab86782)1 mg HeLa whole cell lysate was immunoprecipitated with 10 µg ab86782 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was loaded onto a Western blot and labelled with ab86782 at 1 µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.
