Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [PC10] to PCNA
Suitable for: WB, IP, IHC-P, ICC/IF
Reacts with: Mouse, Human
Isotype: IgG2a

Product name

Anti-PCNA antibody [PC10]
See all PCNA primary antibodies
Description

Mouse monoclonal [PC10] to PCNA
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Rat PCNA.
Database link: P12004
Positive control
- IHC-P: Human colon carcinoma tissue and mouse colon carcinoma tissue. ICC/IF: H226 cells. WB: TCMK-1, NIH/3T3, HeLa and jurkat cell lysate.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

PC10
Isotype

IgG2a
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB
Immunoprecipitation - Anti-PCNA antibody [PC10] (ab265585)

PCNA was immunoprecipitated from 1 mg TCMK-1 cell lysate using ab265585 at 20 µl/mg lysate. Western blot was performed on the immunoprecipitate using ab265585 at 1/1000 dilution.

Lane 1: ab265585 IP in TCMK-1 whole cell lysate.
Lane 2: Control IgG.
Western blot - Anti-PCNA antibody [PC10] (ab265585)

All lanes : Anti-PCNA antibody [PC10] (ab265585) at 1/1000 dilution

Lane 1 : TCMK-1 whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP conjugated goat anti-mouse IgG

Developed using the ECL technique.

Predicted band size: 29 kDa

Exposure time: 1 second
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [PC10] (ab265585)

Formalin-fixed, paraffin-embedded H226 cells stained for PCNA using ab265585 at 1/100 dilution in ICC/IF analysis. Substrate: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab265585)

Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com