Anti-PCNA antibody [PC10] (ab265585)
Key features and details
- Mouse monoclonal [PC10] to PCNA
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-PCNA antibody [PC10]
See all PCNA primary antibodies -
Description
Mouse monoclonal [PC10] to PCNA -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanIP MouseWB Mouse -
Immunogen
Recombinant full length protein corresponding to Rat PCNA.
Database link: P12004 -
Positive control
- IHC-P: Human colon carcinoma tissue and mouse colon carcinoma tissue. ICC/IF: H226 cells. WB: TCMK-1, NIH/3T3, HeLa and jurkat cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
PC10 -
Isotype
IgG2a -
Research areas
Images
-
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB
-
PCNA was immunoprecipitated from 1 mg TCMK-1 cell lysate using ab265585 at 20 µl/mg lysate. Western blot was performed on the immunoprecipitate using ab265585 at 1/1000 dilution.
Lane 1: ab265585 IP in TCMK-1 whole cell lysate.
Lane 2: Control IgG. -
All lanes : Anti-PCNA antibody [PC10] (ab265585) at 1/1000 dilution
Lane 1 : TCMK-1 whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 1 second
-
Formalin-fixed, paraffin-embedded H226 cells stained for PCNA using ab265585 at 1/100 dilution in ICC/IF analysis. Substrate: DAB.
-
Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB