Anti-PCNA antibody [EPR3822] (ab92729)
![Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.com/ps/products/92/ab92729/Images/ab92729-107938-anti-pcna-antibody-epr3822-immunohistochemistry.jpg "Anti-PCNA antibody [EPR3822] (ab92729)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3822] to PCNA
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PCNA antibody [EPR3822]
See all PCNA primary antibodies -
Description
Rabbit monoclonal [EPR3822] to PCNA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human PCNA aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, HepG2 and 293T cell lysates; Human colonic adenocarcinoma tissue; Human tonsil tissue
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3822 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)
ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemisty.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.com/ps/products/92/ab92729/Images/ab92729-107937-anti-pcna-antibody-epr3822-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human tonsil tissue by Immunohistochemisty.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.com/ps/products/92/ab92729/Images/ab92729-107939-anti-pcna-antibody-epr3822-western-blot.jpg)
Western blot - Anti-PCNA antibody [EPR3822] (ab92729)All lanes : Anti-PCNA antibody [EPR3822] (ab92729) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 29 kDa
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![Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.com/ps/products/92/ab92729/Images/ab92729-4-benefits-of-recombinant-antibodies.png)
Anti-PCNA antibody [EPR3822] (ab92729)
