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Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [Hermes-3] to CD44 - BSA and Azide free
  • Suitable for: ELISA, IHC-P, WB
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-CD44 antibody [Hermes-3] - BSA and Azide free
    See all CD44 primary antibodies

  • Description

    Mouse monoclonal [Hermes-3] to CD44 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant fragment corresponding to Human CD44.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: A549 and HeLa whole cell lysate. IHC-P: Human skin tissue. Human bladder carcinoma tissue.

  • General notes

    ab255946 is the PBS only version of ab254530.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)
    Western blot - Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)
    All lanes : Anti-CD44 antibody [Hermes-3] (ab254530) at 0.536 µg/ml

    Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 81 kDa


    Exposure time: 1 second


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab254530).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab254530).

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab254530 at 0.107µg/ml, followed by ready to use secondary. Membranous staining on human skin is observed. The section was incubated with ab254530 for 5 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab254530).

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling CD44 with ab254530 at 0.107µg/ml, followed by ready to use secondary. Membranous staining on human bladder carcinoma is observed. The section was incubated with ab254530 for 5 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • ELISA - Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)
    ELISA - Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab254530).

    ELISA - Anti-CD44 antibody [Hermes-3] (ab254530).

    Antigen: Human CD44.

    ab254530 used at 1000-0 ng/ml.

    Secondary is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.

  • Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)
    Anti-CD44 antibody [Hermes-3] - BSA and Azide free (ab255946)

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