Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1013Y] to CD44 - Low endotoxin, Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free
    See all CD44 primary antibodies

  • Description

    Rabbit monoclonal [EPR1013Y] to CD44 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-231, TF-1, HeLa and A549 cell lysates. Flow Cyt: MDA-MB-231 cells. IHC-P: Pancreatic and cervival cancer, breast and thyroid gland carcinoma, glioma, tonsil and skin tissues.

  • General notes

    ab246690 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Flow Cytometry - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

    ab51037 used at a 1/200 dilution (red) to detect CD44 in human MDA-MB-231 cells by Flow Cytometry. The secondary used was a Pgoat anti-rabbit IgG (Alexa Fluor® 488) polyclonal used at a 1/2000 dilution. Isotype control (black): Rabbit monoclonal IgG (ab172730). Unlabelled control (Blue): Cell without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).

  • Western blot - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Western blot - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    All lanes : Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/5000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CD44 knockout HeLa cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : LnCAP cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 82 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab51037).

    Lanes 1 - 4: Merged signal (red and green). Green - ab51037 observed at 80 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab51037 was shown to react with CD44 in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab51037 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

    ab51037 (1:100) showing positive staining in human Breast carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

    ab51037 (1:100) showing positive staining in human Glioma tissue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

    ab51037 (1:100) showing positive staining in human Thyroid gland carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    OI-RD Scanning - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).

  • Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
    Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)

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