Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3190(2)] to Laminin beta 1
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Laminin beta 1 antibody [EPR3190(2)]
    See all Laminin beta 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3190(2)] to Laminin beta 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney, HeLa and A431 lysates. Flow Cyt: A431 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)
    Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)
    All lanes : Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LAMB1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 198 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab109293 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

     ab109293 was shown to react with Laminin beta 1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265414 (knockout cell lysate ab257499) was used. Wild-type HeLa and LAMB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109293 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)
    Flow Cytometry - Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)
    Flow cytometric analysis of permeabilized A431 cells using ab109293 (red) at a diluiton of 1/100 or a rabbit IgG (negative) (green).
  • Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)
    Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)
    All lanes : Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : A431 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 198 kDa

  • Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)
    Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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