Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3190(2)] to Laminin beta 1
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-Laminin beta 1 antibody [EPR3190(2)]
See all Laminin beta 1 primary antibodies
Description

Rabbit monoclonal [EPR3190(2)] to Laminin beta 1
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal kidney, HeLa and A431 lysates. Flow Cyt: A431 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3190(2)
Isotype

IgG
Research areas

Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)

All lanes : Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LAMB1 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 198 kDa
Observed band size: 200 kDa
why is the actual band size different from the predicted?

Lanes 1- 2: Merged signal (red and green). Green - ab109293 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

ab109293 was shown to react with Laminin beta 1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265414 (knockout cell lysate ab257499) was used. Wild-type HeLa and LAMB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109293 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)

Flow cytometric analysis of permeabilized A431 cells using ab109293 (red) at a diluiton of 1/100 or a rabbit IgG (negative) (green).
Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)

All lanes : Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293) at 1/1000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : A431 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 198 kDa
Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293)

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