Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)
Key features and details
- Mouse monoclonal [CL3183] to Laminin alpha 4/LAMA4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Laminin alpha 4/LAMA4 antibody [CL3183]
See all Laminin alpha 4/LAMA4 primary antibodies -
Description
Mouse monoclonal [CL3183] to Laminin alpha 4/LAMA4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB Human -
Immunogen
Recombinant fragment corresponding to Human Laminin alpha 4/LAMA4 aa 650-850.
Database link: Q16363 -
Epitope
Binds to an epitope located within the peptide sequence MANNLTNWSQNLQHF as determined by overlapping synthetic peptides. -
Positive control
- WB: Human placenta lysate. Lam-411 and Lam-421 purified recombinant human proteins. IHC-P: Human colon, pancreas, placenta and prostate tissue. Rat heart muscle, kidney, epididymis, rectum and stomach tissue. Mouse epididymis and stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CL3183 -
Isotype
IgG2b -
Research areas
Images
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Formalin-fixed, paraffin-embedded rat epididymis tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded mouse epididymis tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded mouse stomach tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat rectum tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat kidney tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat heart muscle tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat stomach tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Negative control - Formalin-fixed, paraffin-embedded human lymph node tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198) at 1 µg/ml + Human placenta tissue lysate
Predicted band size: 202 kDa
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All lanes : Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198) at 1 µg/ml
Lane 1 : Recombinant human Laminin-411 protein
Lane 2 : Recombinant human Laminin-421 protein
Lane 3 : Recombinant human Laminin-511 protein
Lane 4 : Recombinant human Laminin-121 protein
Lane 5 : Recombinant human Laminin-221 protein
Lane 6 : Recombinant human Laminin-332 protein
Predicted band size: 202 kDa