Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Key features and details

Mouse monoclonal [CL3183] to Laminin alpha 4/LAMA4
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human
Isotype: IgG2b

Product name

Anti-Laminin alpha 4/LAMA4 antibody [CL3183]
See all Laminin alpha 4/LAMA4 primary antibodies
Description

Mouse monoclonal [CL3183] to Laminin alpha 4/LAMA4
Host species

Mouse

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Laminin alpha 4/LAMA4 aa 650-850.
Database link: Q16363

Run BLAST with Run BLAST with
Epitope

Binds to an epitope located within the peptide sequence MANNLTNWSQNLQHF as determined by overlapping synthetic peptides.
Positive control
- WB: Human placenta lysate. Lam-411 and Lam-421 purified recombinant human proteins. IHC-P: Human colon, pancreas, placenta and prostate tissue. Rat heart muscle, kidney, epididymis, rectum and stomach tissue. Mouse epididymis and stomach tissue.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

CL3183
Isotype

IgG2b
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded rat epididymis tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded mouse epididymis tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded mouse stomach tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded rat rectum tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded rat kidney tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded rat heart muscle tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded rat stomach tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Negative control - Formalin-fixed, paraffin-embedded human lymph node tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Formalin-fixed, paraffin-embedded human colon tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
Western blot - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198) at 1 µg/ml + Human placenta tissue lysate

Predicted band size: 202 kDa
Western blot - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)

All lanes : Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198) at 1 µg/ml

Lane 1 : Recombinant human Laminin-411 protein
Lane 2 : Recombinant human Laminin-421 protein
Lane 3 : Recombinant human Laminin-511 protein
Lane 4 : Recombinant human Laminin-121 protein
Lane 5 : Recombinant human Laminin-221 protein
Lane 6 : Recombinant human Laminin-332 protein

Predicted band size: 202 kDa

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