Anti-Cathepsin K antibody (ab85716)
Key features and details
- Rabbit polyclonal to Cathepsin K
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cathepsin K antibody
See all Cathepsin K primary antibodies -
Description
Rabbit polyclonal to Cathepsin K -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Cathepsin K aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab97980) -
Positive control
- This antibody gave a positive signal in the following lysates: Human Bone Marrow Tissue; Human Lung Tissue; U2OS Whole Cell; MCF7 Whole Cell; MDA-MB-231 Whole Cell; MDA-MB-361 Whole Cell; Saos 2 Whole Cell.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatChickenCowDogPigNon human primatesApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 37 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 37 kDa).Target
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Function
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Tissue specificity
Predominantly expressed in osteclasts (bones). -
Involvement in disease
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 513038 Cow
- Entrez Gene: 1513 Human
- Entrez Gene: 13038 Mouse
- Entrez Gene: 397569 Pig
- Entrez Gene: 29175 Rat
- Omim: 601105 Human
- SwissProt: Q5E968 Cow
- SwissProt: Q3ZKN1 Dog
see all -
Alternative names
- Cathepsin K antibody
- Cathepsin O antibody
- Cathepsin O1 antibody
see all
Images
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Western blot - Anti-Cathepsin K antibody (ab85716)All lanes : Anti-Cathepsin K antibody (ab85716) at 1 µg/ml
Lane 1 : Bone marrow (Human) Tissue Lysate
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
We hypothesize that the 24 kDa band represents the mature form of Cathepsin K.
References (1)
ab85716 has been referenced in 1 publication.
- Maatouk L et al. TLR9 activation via microglial glucocorticoid receptors contributes to degeneration of midbrain dopamine neurons. Nat Commun 9:2450 (2018). PubMed: 29934589
Images
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Western blot - Anti-Cathepsin K antibody (ab85716)All lanes : Anti-Cathepsin K antibody (ab85716) at 1 µg/ml
Lane 1 : Bone marrow (Human) Tissue Lysate
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
We hypothesize that the 24 kDa band represents the mature form of Cathepsin K.
