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Signal Transduction Cytoskeleton / ECM Extracellular Matrix

Anti-Cathepsin K antibody [EP9403] (ab187647)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Cathepsin K antibody [EP9403] (ab187647)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP9403] to Cathepsin K
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cathepsin K antibody [EP9403]
    See all Cathepsin K primary antibodies
  • Description

    Rabbit monoclonal [EP9403] to Cathepsin K
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and rat cartilage lysate, mouse and rat muscle lysates, human skeletal muscle lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP9403
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Neuroscience
    • Cell Adhesion Proteins
    • Proteases
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Cysteine protease
    • Cathepsins
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other

Images

  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    All lanes : Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (purified)

    Lane 1 : Mouse cartilage lysates
    Lane 2 : Rat muscle lysates
    Lane 3 : Mouse muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    All lanes : Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (Purified)

    Lane 1 : Human cartilage lysates
    Lane 2 : Human skeletal muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 37 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/5000 dilution (unpurified) + Rat cartilage lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (unpurified) + Mouse muscle lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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