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Human/Mouse/Rat MEK1 Matched Antibody Pair Kit (ab219523)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse, Rat, Human
  • Range: 31.25 pg/ml - 2000 pg/ml

Overview

  • Product name

    Human/Mouse/Rat MEK1 Matched Antibody Pair Kit
    See all MEK1 kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Sensitivity

    1.4 pg/ml

  • Range

    31.25 pg/ml - 2000 pg/ml

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Product overview

    MEK1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant MEK1.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208988. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    MEK1 Capture Antibody 2 x 50µg 1 x 50µg
    MEK1 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    MEK1 Lyophilized Protein 2 vials 1 vial

  • Research areas

  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

  • Tissue specificity

    Widely expressed, with extremely low levels in brain.

  • Involvement in disease

    Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
    Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt

  • Alternative names

    • Dual specificity mitogen activated protein kinase kinase 1
    • Dual specificity mitogen-activated protein kinase kinase 1
    • ERK activator kinase 1
    • MAP kinase kinase 1
    • MAP kinase/Erk kinase 1
    • MAP2K1
    • MAPK/ERK kinase 1
    • MAPKK 1
    • MAPKK1
    • MEK 1
    • Mek1
    • MEKK1
    • Mitogen activated protein kinase kinase 1
    • MKK 1
    • MKK1
    • MP2K1_HUMAN
    • PRKMK1
    • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1)
    • Protein kinase mitogen activated, kinase 1
    • protein kinase mitogen-activated kinase 1
    see all

  • Database links

    see all

Images

  • Human/Mouse/Rat MEK1 standard curve
    Human/Mouse/Rat MEK1 standard curve

    Standard calibration curve. Background subtracted values are graphed.

  • Sandwich ELISA - Human/Mouse/Rat MEK1 Matched Antibody Pair Kit (ab219523)
    Sandwich ELISA - Human/Mouse/Rat MEK1 Matched Antibody Pair Kit (ab219523)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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