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Anti-PHKG1 antibody [EPR14812] (ab194112)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14812] to PHKG1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PHKG1 antibody [EPR14812]
    See all PHKG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14812] to PHKG1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle, mouse and rat muscle lysates; Human skeletal muscle, Human hepatocellular carcinoma and rat kidney tissues; A673 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112)
    Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112)
    Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/20000 dilution + Human skeletal muscle lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112)
    Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112)
    All lanes : Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/1000 dilution

    Lane 1 : Mouse muscle lysate
    Lane 2 : Rat muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] (ab194112)
    Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] (ab194112)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A673 cells labeling PHKG1 with ab194112 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain is DAPI (blue).

  • Anti-PHKG1 antibody [EPR14812] (ab194112)
    Anti-PHKG1 antibody [EPR14812] (ab194112)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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