Anti-PHKG1 antibody [EPR14812] (ab194112)
![Anti-PHKG1 antibody [EPR14812] (ab194112)](https://www.abcam.com/ps/products/194/ab194112/Images/ab194112-231835-anti-phkg1-antibody-epr14812-immunohistochemistry.jpg "Anti-PHKG1 antibody [EPR14812] (ab194112)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14812] to PHKG1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PHKG1 antibody [EPR14812]
See all PHKG1 primary antibodies -
Description
Rabbit monoclonal [EPR14812] to PHKG1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P RatHumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle, mouse and rat muscle lysates; Human skeletal muscle, Human hepatocellular carcinoma and rat kidney tissues; A673 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14812 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112)](https://www.abcam.com/ps/products/194/ab194112/Images/ab194112-231833-anti-phkg1-antibody-epr14812-western-blot.jpg)
Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112)Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/20000 dilution + Human skeletal muscle lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112)](https://www.abcam.com/ps/products/194/ab194112/Images/ab194112-231834-anti-phkg1-antibody-epr14812-western-blot.jpg)
Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112)All lanes : Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/1000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Rat muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)](https://www.abcam.com/ps/products/194/ab194112/Images/ab194112-231836-anti-phkg1-antibody-epr14812-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)](https://www.abcam.com/ps/products/194/ab194112/Images/ab194112-231837-anti-phkg1-antibody-epr14812-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (ab194112)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling PHKG1 with ab194112 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] (ab194112)](https://www.abcam.com/ps/products/194/ab194112/Images/ab194112-231838-anti-phkg1-antibody-epr14812-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] (ab194112)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A673 cells labeling PHKG1 with ab194112 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain is DAPI (blue).
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![Anti-PHKG1 antibody [EPR14812] (ab194112)](https://www.abcam.com/ps/products/194/ab194112/Images/ab194112-7-benefits-of-recombinant-antibodies.png)
Anti-PHKG1 antibody [EPR14812] (ab194112)