Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MSK1 (phospho S376) antibody [E375] (ab32190)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E375] to MSK1 (phospho S376)
Suitable for: WB, IHC-P, ICC
Reacts with: Human

Product name

Anti-MSK1 (phospho S376) antibody [E375]
See all MSK1 primary antibodies
Description

Rabbit monoclonal [E375] to MSK1 (phospho S376)
Host species

Rabbit
Specificity

This antibody will detect MSK1 phosphorylation on Serine 376.

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human MSK1. The exact sequence is proprietary.
Positive control
- WB: K562 cell lysate. IHC-P Human urinary bladder carcinoma. ICC: HepG2 cells
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Clonality

Monoclonal
Clone number

E375
Isotype

IgG
Research areas

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)

All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

Lane 1 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates
Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 (phosphor S376) peptide
Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 non-phospho peptide

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 90 kDa

Exposure time: 10 seconds

Blocking/Diluting buffer: 5% NFDM/TBST

Observed band: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.
Immunocytochemistry - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)

Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling MSK1 (phospho S376) with ab32190 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 AlexaFluor^®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as Nuclear counterstain.

Confocal image showing decreased nuclear staining in HepG2 cells treated with Alkaline Phosphatase (37℃ 1h).
Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)

All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

Lane 1 : Untreated HEK293 (human embryonic kidney) whole cell lysates
Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysates
Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 90 kDa

Exposure time: 5 seconds

Blocking/Diluting buffer 5% NFDM/TBST.

Observed band: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.
Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)

All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilution

Lane 1 : Untreated K562 cell lysate
Lane 2 : K562 cell lysate treated with Alkaline

Predicted band size: 90 kDa
Observed band size: 87 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)

Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-MSK1 (phospho S376) antibody [E375] (ab32190)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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