Glycogen Phosphorylase Assay Kit (Colorimetric) (ab273271)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Glycogen Phosphorylase Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Glycogen Phosphorylase Assay Kit (Colorimetric) (ab273271) is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at 450 nm. The OD 450 nm signal is directly proportional to the glycogen phosphorylase activity. The Glycogen Phosphorylase Colorimetric Assay Kit is rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity. The kit can detect as low as 10 mU in a variety of sample types.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer 1 x 20ml Developer (Lyophilized) 1 vial Enzyme Mix (Lyophilized) 1 vial G1P Standard (Lyophilized) 1 vial Glycogen (Lyophilized) 1 vial Glycogen Phosphorylase (Lyophilized) 1 vial Substrate Mix (Lyophilized) 1 vial
Images
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G1P Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
G1P Standard Curve.
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Reaction time-courseReaction curves of Glycogen Phosphorylase versus no enzyme control.
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Glycogen phosphorylase activity in different sample types
