Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)
![Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)](https://www.abcam.com/ps/products/81/ab81294/Images/MSK1-Primary-antibodies-ab81294-1.JPG "Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)")
Key features and details
- Rabbit monoclonal [EP1888Y] to MSK1 (phospho S360)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MSK1 (phospho S360) antibody [EP1888Y]
See all MSK1 primary antibodies -
Description
Rabbit monoclonal [EP1888Y] to MSK1 (phospho S360) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HEK293 cell lyasate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1888Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)All lanes : Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294) at 1/20000 dilution
Lane 1 : HEK293 cell lyasate, untreated,
Lane 2 : HEK293 cell lyasate treated with AP.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit. at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)](https://www.abcam.com/ps/products/81/ab81294/Images/MSK1-Primary-antibodies-ab81294-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)ICC/IF image of ab81294 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81294, neat) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
