Anti-MSK1 (phospho S212) antibody (ab79499)
Key features and details
- Rabbit polyclonal to MSK1 (phospho S212)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MSK1 (phospho S212) antibody
See all MSK1 primary antibodies -
Description
Rabbit polyclonal to MSK1 (phospho S212) -
Host species
Rabbit -
Specificity
ab79499 detects endogenous levels of MSK1 only when phosphorylated at serine 212 -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of serine 212 (AYSPFC)
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Positive control
- IHC: human breast carcinoma tissue WB: extracts from 3T3 cells (treated with EGF at 200 ng/ml for 5mins)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MSK1 (phospho S212) antibody (ab79499)All lanes : Anti-MSK1 (phospho S212) antibody (ab79499) at 1/1000 dilution
Lane 1 : extracts from 3T3 cells (treated
with EGF at 200ng/ml for 5 mins)
Lane 2 : extracts from 3T3 cells (treated
with EGF at 200ng/ml for 5 mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S212) antibody (ab79499)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, stained with ab79499, at 1/100 dilution, in the absence and presence of the immunising peptide
