Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)
![Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)](https://www.abcam.com/ps/products/247/ab247249/Images/ab247249-405391-anti-caspase-6-casp-6-antibody-e180-bsa-and-azide-free-western-blot.jpg "Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E180] to Caspase-6/CASP-6 - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free
See all Caspase-6/CASP-6 primary antibodies -
Description
Rabbit monoclonal [E180] to Caspase-6/CASP-6 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects the pro form and the p11 subunit of human Caspase-6/CASP-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
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Tested applications
Suitable for: IHC-P, ICC, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247249 is the carrier-free version of ab32366. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
E180 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)All lanes : Anti-Caspase-6/CASP-6 antibody [E180] (ab32366) at 1/1000 dilution
Lane 1 : Untreated Jurkat cell lysate
Lane 2 : Jurkat cell lysate treated with Camptothecin.
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?This data was developed using ab32366, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)](https://www.abcam.com/ps/products/247/ab247249/Images/ab247249-2-anti-caspase-6-casp-6-antibody-e180-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)This data was developed using ab32366, the same antibody clone in a different buffer formulation.Ab32366, at a 1:50 dilution, staining Caspase-6/CASP-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)](https://www.abcam.com/ps/products/247/ab247249/Images/ab247249-1-anti-caspase-6-casp-6-antibody-e180-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)This data was developed using ab32366, the same antibody clone in a different buffer formulation.Ab32366, at a 1:100 dilution, staining Caspase-6/CASP-6 in Hela cells by Immunocytochemistry/Immunofluorescence. -
![Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)](https://www.abcam.com/ps/products/247/ab247249/Images/ab247249-4-benefits-of-recombinant-antibodies.png)
Anti-Caspase-6/CASP-6 antibody [E180] - BSA and Azide free (ab247249)