Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µl 268 032 ₸

Order now and get it on Tuesday March 16, 2021

Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2293Y] to Hsp27 (phospho S15)
Suitable for: WB, ICC/IF
Reacts with: Human

Product name

Anti-Hsp27 (phospho S15) antibody [EP2293Y]
See all Hsp27 primary antibodies
Description

Rabbit monoclonal [EP2293Y] to Hsp27 (phospho S15)
Host species

Rabbit
Specificity

ab76313 will detect Hsp27 phosphorylation on Serine 15.
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Serine 15 of human Hsp27.
Positive control
- HeLa cells, HeLa cell lysate heat treated at 44°C.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.13% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2293Y
Isotype

IgG
Research areas

Western blot - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)

All lanes : Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313) at 1/2500 dilution

Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 23 kDa
Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)

ab76313 staining Hsp27 (phospho S15) in HeLa (human cervix adenocarcinoma) cells with heat treatment at 44°C. by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab76313 (1/1000) and secondary antibody ab150077 (1/1000) respectively and DAPI was used as a nuclear counterstain.

Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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