Anti-Hsp27 (phospho S82) antibody (ab17937)
Key features and details
- Rabbit polyclonal to Hsp27 (phospho S82)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp27 (phospho S82) antibody
See all Hsp27 primary antibodies -
Description
Rabbit polyclonal to Hsp27 (phospho S82) -
Host species
Rabbit -
Specificity
When tested by Western blotting on TNF alpha stimulated HeLa lysates the antibody detects a single clean band of about 27kD. This band is only blocked by the phospho peptide immunogen and not by an equivalent non-phospho peptide or by a generic phospho peptide. This confirms that the antibody is specific for phospho S82 of Hsp27. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho peptide (Human).
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Positive control
- HeLa cells treated with TNF alpha.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated HSP27. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hsp27 (phospho S82) antibody (ab17937)
Western blot using ab17937 on HeLa cell lysate.
Lane 1: Unstimulated HeLa lysate
Lane 2: TNF-α Stimulated HeLa lysate
Lane 3: TNF-α Stimulated HeLa lysate blocked with non-phospho peptide (equivalent to immunogen sequence)
Lane 4: TNF-α Stimulated HeLa lysate blocked with generic phospho-serine peptide.
Lane 5: TNF-α Stimulated HeLa lysate blocked with phosphopeptide immunogen.10-30
µ g of cell lysate can be loaded when using similar lysates with this antibody. Samples were run using SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with ab17937 for one hour at room temperature in 3% BSA-TBST buffer, following prior incubation with blocking peptides. After washing, membranes were incubated with goat F(ab')2 anti
