Anti-Hsp90 beta antibody [K3701] (ab82584)
![Anti-Hsp90 beta antibody [K3701] (ab82584)](https://www.abcam.com/ps/products/82/ab82584/Images/ab82584-388318-anti-hsp90-beta-antibody-k3701-western-blot-a431-wt-a431-hsp90ab1-knockout-saos2-hl60.jpg "Anti-Hsp90 beta antibody [K3701] (ab82584)")
Key features and details
- Mouse monoclonal [K3701] to Hsp90 beta
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Rat, Human, Chinese hamster, Recombinant fragment
- Isotype: IgM
Overview
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Product name
Anti-Hsp90 beta antibody [K3701]
See all Hsp90 beta primary antibodies -
Description
Mouse monoclonal [K3701] to Hsp90 beta -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant human Hsp90 beta protein
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Positive control
- WB: A431, Saos-2, HL-60, HeLa, PC12, CHO-K1 cell lysates; Hsp90 native protein. Flow Cyt: Human colon cancer Caco-2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6.90
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
K3701 -
Isotype
IgM -
Research areas
Images
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Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)All lanes : Anti-Hsp90 beta antibody [K3701] (ab82584) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : HSP90AB1 knockout A431 cell lysate
Lane 3 : Saos-2 cell lysate
Lane 4 : HL-60 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab82584 observed at 85 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab82584 was shown to react with Hsp90 beta in wild-type A431 cells in western blot. Loss of signal was observed when HSP90AB1 knockout sample was used. Wild-type A431 and HSP90AB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab82584 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)](https://www.abcam.com/ps/products/82/ab82584/Images/Hsp90-beta-Primary-antibodies-ab82584-1.jpg)
Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)All lanes : Anti-Hsp90 beta antibody [K3701] (ab82584) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : CHO-K1 cell lysate
Lane 4 : Hsp90 Native Protein
Lane 5 : Hsp90 alpha Recombinant Protein (negative control)
Predicted band size: 83 kDa
Observed band size: 83 kDa
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![Flow Cytometry - Anti-Hsp90 beta antibody [K3701] (ab82584)](https://www.abcam.com/ps/products/82/ab82584/Images/Hsp90-beta-Primary-antibodies-ab82584-3.jpg)
Flow Cytometry - Anti-Hsp90 beta antibody [K3701] (ab82584)ab82584, at 10 µg/ml, staining p90 beta in human colon cancer Caco-2 cells by flow cytometry (right). Isotype control antibody (left).
