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Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23549-163] to Superoxide Dismutase 1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free
    See all Superoxide Dismutase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23549-163] to Superoxide Dismutase 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7, A431 and Human brain, Human placenta lysates. IHC-P: Human kidney tissue. ICC/IF: MCF7 cell. Flow Cyt: MCF7 cell. IP: MCF7 cell.

  • General notes

    ab272048 is the carrier-free version of ab252426. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Superoxide dismutase 1 with ab252426 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney (PMID: 29861522). The section was incubated with ab252426 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252426).

  • Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)
    Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 cells labelling Superoxide dismutase 1 with ab252426 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic and nuclear staining in MCF7 cell line Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252426).

  • Flow Cytometry - Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)
    Flow Cytometry - Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Superoxide dismutase 1 with ab252426 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252426).

  • Immunoprecipitation - Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)
    Immunoprecipitation - Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)

    Superoxide dismutase 1 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab252426 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252426 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab252426 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252426 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252426).

  • Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)
    Anti-Superoxide Dismutase 1 antibody [EPR23549-163] - BSA and Azide free (ab272048)

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