Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free
    See all Superoxide Dismutase 1 primary antibodies

  • Description

    Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Mouse
    ICC/IF
    Human
    IHC-P
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebrum, rat liver and human breast carcinoma tissue WB: Jurkat cell lysate; Mouse and rat brain tissue lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Ab238954 is the carrier-free version of ab51254. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238954 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)
    Immunocytochemistry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)

    This data was developed using ab51254, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Superoxide Dismutase 1 with ab51254 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in NIH/3T3 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

  • Immunocytochemistry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)
    Immunocytochemistry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)

    This data was developed using ab51254, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Superoxide Dismutase 1 with ab51254 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51254)
  • Flow Cytometry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)
    Flow Cytometry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Superoxide Dismutase 1 with ab51254 at 1/20 dilution (1 µg) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51254).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51254)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)

    Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51254).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)
    Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)

    Immunofluorescent staining of HeLa (human epithelial cell line from cervix adenocarcinoma) cells using ab51254 (1:100).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51254).

  • Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)
    Anti-Superoxide Dismutase 1 antibody [EP1727Y] - BSA and Azide free (ab238954)

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