Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19202-244] to Claudin18.2 - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free
    See all Claudin18.2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19202-244] to Claudin18.2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human stomach and pancreatic cancer tissue; mouse stomach tissue; rat stomach tissue. IHC-Fr: Mouse stomach tissue; rat stomach tissue.

  • General notes

    Ab242079 is the carrier-free version of ab241330. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242079 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19202-244

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

    Immunohistochemical analysis of paraffin-embedded rat lung (A) and stomach (B) tissue labeling Claudin18.2 with ab241330 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on rat lung (negative control), but membranous staining on rat gastric epithelial cells (PMID: 19047087) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241330).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

    Immunohistochemical analysis of paraffin-embedded mouse lung (A) and stomach (B) tissue labeling Claudin18.2 with ab241330 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on mouse lung (negative control), but membranous staining on mouse gastric epithelial cells (PMID: 19047087) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241330).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

    Immunohistochemical analysis of paraffin-embedded human normal pancreas (A) and pancreatic cancer (B) tissue labeling Claudin18.2 with ab241330 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on human normal pancreas (negative control), but membranous staining on human pancreas cancer cells (PMID: 21832145) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241330).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

    Immunohistochemical analysis of paraffin-embedded human lung (A) and stomach (B) tissue labeling Claudin18.2 with ab241330 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on human lung (negative control), but membranous staining on human gastric epithelial cells (PMID: 19047087) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241330).

  • Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)
    Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat lung tissue labeling Claudin18.2 with ab241330 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Negative control:No staining on mouse lung (PMID: 19047087) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241330).

  • Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)
    Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat stomach tissue labeling Claudin18.2 with ab241330 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Membranous staining on rat stomach is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241330).

  • Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)
    Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse lung tissue labeling Claudin18.2 with ab241330 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Negative control:No staining on mouse lung (PMID: 19047087) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241330).

  • Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)
    Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse stomach tissue labeling Claudin18.2 with ab241330 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Membranous staining on mouse stomach is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241330).

  • Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)
    Anti-Claudin18.2 antibody [EPR19202-244] - BSA and Azide free (ab242079)

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