Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19202] to Claudin18.2 - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free
    See all Claudin18.2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19202] to Claudin18.2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240400 is the carrier-free version of ab222512. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240400 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19202

  • Isotype

    IgG

Images

  • Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

    Immunohistochemical analysis of OCT-embedded mouse stomach tissue labeling Claudin18.2 with ab222512 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cell membrane staining on gastric mucosa epithelial cells (PMID: 21571049). Nuclear counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222512).

  • Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Immunohistochemistry (Frozen sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

    Immunohistochemical analysis of OCT-embedded rat stomach tissue labeling Claudin18.2 with ab222512 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cell membrane staining on gastric mucosa epithelial cells (PMID: 21571049). Nuclear counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222512).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Claudin18.2 with ab222512 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human gastric epithelial cells is observed (PMID: 21571049). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222512).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Claudin18.2 with ab222512 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. We observed membranous staining on gastric cancer cells (PMID: 19047087). It has been documented in the literature that Claudin18.2 can be up-regulated or down-regulated in certain gastric cancers. (PMID: 19047087; PMID: 16435283). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222512).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Claudin18.2 with ab222512 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on adjacent normal gastric epithelial cells but negative staining on stomach cancer cells (PMID: 16435283). It has been documented in the literature that Claudin18.2 can be up-regulated or down-regulated in certain gastric cancers. (PMID: 19047087; PMID: 16435283). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222512).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Claudin18.2 with ab222512 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on colon cancer cells is observed (PMID: 19047087). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222512).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

    Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Claudin18.2 with ab222512 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative control: No staining on human lung is observed (PMID21571049). Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222512).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Claudin18.2 with ab222512 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on mouse gastric epithelial cells is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222512).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Claudin18.2 with ab222512 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on rat gastric epithelial cells is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222512).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)
    Anti-Claudin18.2 antibody [EPR19202] - BSA and Azide free (ab240400)

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