Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2324Y] to ErbB2 / HER2 (phospho Y877) - BSA and Azide free
  • Suitable for: ICC, Dot blot, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free
    See all ErbB2 / HER2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2324Y] to ErbB2 / HER2 (phospho Y877) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects ErbB 2 phosphorylated at tyrosine 877.

  • Tested applications

    Suitable for: ICC, Dot blot, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247413 is the carrier-free version of ab75873 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247413 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)
    Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)
    All lanes : Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (ab75873) at 1/1000 dilution

    Lane 1 : HeLa cell lysate, untreated
    Lane 2 : HeLa cell lysate, treated with EGF
    Lane 3 : SKBR-3 cell lysate, untreated
    Lane 4 : SKBR-3 cell lysate, treated with EGF

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 138 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab75873, the same antibody clone in a different buffer formulation.

  • Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)
    Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)

    This data was developed using ab75873, the same antibody clone in a different buffer formulation.Dot blot analysis of ErbB 2 (pY877) peptide (Lane 1) and ErbB2 / HER2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y877) with ab75873 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.

  • Flow Cytometry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)
    Flow Cytometry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)
    This data was developed using ab75873, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB 2 with unpurified ab75873 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Immunocytochemistry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)
    Immunocytochemistry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)

    This data was developed using ab75873, the same antibody clone in a different buffer formulation.ab75873 at 1/50 dilution staining ErbB2 / HER2 in SKBR3 cells by Immunofluorescence.

  • OI-RD Scanning - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)
    OI-RD Scanning - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)
    This data was developed using ab75873, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)
    Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)

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