Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)
![Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)](https://www.abcam.com/ps/products/250/ab250748/Images/ab250748-447338-anti-rcn1-rcn-antibody-epr19193-bsa-and-azide-free-western-blot.jpg "Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19193] to RCN1/RCN - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free
See all RCN1/RCN primary antibodies -
Description
Rabbit monoclonal [EPR19193] to RCN1/RCN - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250748 is the carrier-free version of ab184441 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250748 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as RCN1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19193 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 44,46 kDa why is the actual band size different from the predicted?This data was developed using ab184441, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-3: 30 seconds; Lane 4: 3 minutes.
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![Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)](https://www.abcam.com/ps/products/250/ab250748/Images/ab250748-447313-anti-rcn1-rcn-antibody-epr19193-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : E14 mouse embryo lysate
Lane 5 : E14 rat embryo lysate
Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 7 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 8 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 9 : Rat cerebral cortex lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 44,46 kDa why is the actual band size different from the predicted?This data was developed using ab184441, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-6: 8 seconds; Lanes 7 and 8: 30 seconds; Lane 9: 3 minutes.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8416973).
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![Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)](https://www.abcam.com/ps/products/250/ab250748/Images/ab250748-447285-anti-rcn1-rcn-antibody-epr19193-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : Rat spleen lysate
Lane 7 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 44,46 kDa why is the actual band size different from the predicted?This data was developed using ab184441, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-6: 3 minutes Lanes 7 and 8: 30 seconds; Lanes 9 and 10: 1 second.
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![Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)](https://www.abcam.com/ps/products/250/ab250748/Images/ab250748-6-benefits-of-recombinant-antibodies.png)
Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)