Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19799] to CRISPR-Cas9 - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Staphylococcus aureus

Overview

  • Product name

    Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free
    See all CRISPR-Cas9 primary antibodies

  • Description

    Rabbit monoclonal [EPR19799] to CRISPR-Cas9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Staphylococcus aureus

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells transfected with S.aureus Cas9 (pcDNA3.1(+)-Myc-His).

  • General notes

    Ab218654 is the carrier-free version of ab203933. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab218654 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19799

  • Isotype

    IgG

Images

  • Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Immunocytochemical analysis of agarose-embedded HeLa (Human epithelial cell line from cervix adenocarcinoma) cells transfected with blank pcDNA3.1(+)-Myc-His vector labeling CRISPR-Cas9 with ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
    No staining on HeLa cells transfected with blank pcDNA3.1(+)-Myc-His vector.
    Counter stained with Hematoxylin.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203933).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling CRISPR-Cas9 with ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    No staining on Human endometrium is observed.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203933).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CRISPR-Cas9 with ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    No staining on mouse spleen is observed.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203933).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CRISPR-Cas9 with ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    No staining on rat spleen is observed.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203933).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human epithelial cell line from embryonic kidney) cells transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag or Empty vector, labeling CRISPR-Cas9 with ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Confocal image showing positive staining on 293T cells transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag. 

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab203933 at 1/500 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203933).

  • Flow Cytometry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Flow Cytometry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (Human epithelial cell line from embryonic kidney) cells transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag labeling CRISPR-Cas9 with ab203933 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] -Isotype control (ab172730) (black). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203933).

  • Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

    CRISPR-Cas9 was immunoprecipitated from 0.35 mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag with ab203933 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab203933 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293 whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag 10µg (Input).

    Lane 2: ab203933 IP in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab203933 in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203933).

  • Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human epithelial cell line from embryonic kidney) cells transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag or Empty vector, labeling CRISPR-Cas9 with ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Confocal image showing positive staining on 293T cells transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag. 

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab203933 at 1/500 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203933).

  • Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

    Immunocytochemical analysis of agarose-embedded 293T (Human epithelial cell line from embryonic kidney) cells transfected with Staphylococcus aureus subsp. Aureus Cas9 (pcDNA3.1(+)-Myc-His) labeling CRISPR-Cas9 with ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Positive staining on 293T cells transfected with Staphylococcus aureus subsp. Aureus Cas9 (pcDNA3.1(+)-Myc-His) is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203933).

  • Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
    Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)

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