Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19795] to CRISPR-Cas9
Suitable for: IP, Flow Cyt, ICC/IF, WB
Reacts with: Staphylococcus aureus

Product name

Anti-CRISPR-Cas9 antibody [EPR19795]
See all CRISPR-Cas9 primary antibodies
Description

Rabbit monoclonal [EPR19795] to CRISPR-Cas9
Host species

Rabbit
Tested applications

Suitable for: IP, Flow Cyt, ICC/IF, WBmore details
Species reactivity

Reacts with: Staphylococcus aureus
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T whole cell lysate transfected with Myc-His tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus) vector. ICC/IF: HEK-293T cells transfected with GFP-tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus) expression construct. Flow Cyt: HEK-293T cells transfected with GFP-tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus) expression construct. IP: HEK-293T cell lysate transfected with GFP-tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus) vector.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19795
Isotype

IgG

Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CRISPR-Cas9 with ab203943 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) expression construct.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943)

All lanes : Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943) at 1/5000 dilution

Lane 1 : HEK-293T whole cell lysate (Human epithelial cell line from embryonic kidney) transfected with an empty vector containing Myc-His tag (vector control)
Lane 2 : HEK-293T whole cell lysate transfected with a Myc-His tagged CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)) vector
Lane 3 : HEK-293T whole cell lysate transfected with a Myc-His tagged CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) vector
Lane 4 : HEK-293T whole cell lysate transfected with a Myc-His tagged CRISPR-Cas9 (Q03JI6, Streptococcus thermophiles (strain ATCC BAA-491/ LMD-9)) vector
Lane 5 : HEK-293T whole cell lysate transfected with a Myc-His tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 124 kDa
Observed band size: 124 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943)

Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector, labeling CRISPR-Cas9 with ab203943 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black). Goat anti rabbit IgG (Alexa Fluor^® 647) at 1/2000 dilution was used as the secondary antibody.

Note: The image is obtained by gating the GFP-tag positive population.
Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943)

CRISPR-Cas9 was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector, with ab203943 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203943 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HEK-293T whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector, 10 μg (Input).

Lane 2: ab203943 IP in HEK-293T whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203943 in HEK-293T whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943)

All lanes : Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943) at 1/5000 dilution

Lane 1 : HEK-293 whole cell lysate (Human epithelial cell line from embryonic kidney) transfected with a Myc-His tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : Rat embryo lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 124 kDa
Observed band size: 124 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943)

All lanes : Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943) at 1/5000 dilution

Lane 1 : HEK-293T whole cell lysate (Human epithelial cell line from embryonic kidney transfected with an empty vector containing GFP-Myc tag (vector control)
Lane 2 : HEK-293T whole cell lysate transfected with a GFP-Myc tagged CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) vector
Lane 3 : HEK-293T whole cell lysate transfected with a GFP-Myc tagged CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, aa1-800) vector
Lane 4 : HEK-293 whole cell lysate transfected with a GFP-Myc tagged CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, aa801-1409) vector

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 124 kDa
Observed band size: 124 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943)

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